淀粉碘络合物定量碘的方法.pdf

Carbohydrate Research 345 (2010) 2698–2704 Contents lists available at ScienceDirect Carbohydrate Research journal homepage: www.else /locate/carres A new method for quantifying iodine in a starch–iodine matrix Bruce A. Manion, Bruce E. Holbein, Massimo F. Marcone, Koushik Seetharaman ⇑ Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1 a r t i c l e i n f o a b s t r a c t Article history: A rapid and sensitive method for quantifying iodine in intact starch granules using gas chromatography is Received 16 June 2010 described with detection limits as low as 0.2% (w/w) iodine in starch. Sample preparation includes NaBH4 Received in revised form 8 September 2010 reduction of the various iodine species associated with starch to the colorless soluble iodide ion, followed Accepted 20 September 2010 þ by its quantitative derivatization to EtI using Et3 O BF in CH Cl . Identification and quantification of EtI Available online 1 October 2010 4 2 2 is carried out by extraction and injection of the EtI so generated in CH2Cl2 into a gas chromatography– mass spectrometer (GC–MS). Routine quantification of EtI was then performed using GC with a flame ion- Keywords: ization detector (GC–FID).