P-选择素靶向超声微泡与普通超声微泡粘附途径和效能的对比研究.pdf

·289· ·实验研究· P一选择素靶向超声微泡与普通超声微泡粘附途径和 效能的对比研究 纪丽景杨莉 吴爵非 胡广全谢佳佳 李军华 燕翼宾建平 摘 要 目的荧光显微镜直视下对比评价P一选择素靶向超声微泡与普通超声微泡在炎症内皮上的粘附途径 静脉弹丸式分别注入对照组(10只)和肿瘤坏死因子(田盯一a)处理组(10只)的小鼠提睾肌炎症模型。同时，20高倍荧光 显微镜观测5min内两组不同微泡在提睾肌微血管中的粘附数量和粘附途径。结果iF—a处理组MBp粘附数量为 (12±2．6)个／视野，而MB粘附数量为(3 27．4)％。结论与MB比较，MBp能高效、特异地粘附于炎症组织血管内皮上，应用其可有效评价血管内皮炎症反应或 其他组织损伤。 关键词 超声检查；微气泡，靶向；P一选择素；荧光显微镜 ofal悯oll and arlem,h咖咖p-础蛐targeted C啊珥皿蜘study imllnsays ultrasoundmierolml蜘andcontrastn面既Ⅵbu枞够 regular JIIjjing，YANGLi，WUJuefei，HUGIlan卿l跏，XIEJiaji且，LIJImhlm，YANYi，BINJianping of Medical DeparmaentCardiology，NanfangHospital，SouthernUniversity，GI旧啦丑剐510515，China ABSTRACT Toe、耐ualetheadhesion and ofP—selectinuhmsotmdmicrobubblesand Objective efficacy targeted pathways corfaastmicrobubblesonthe endotheliumbv The micmbubbleswith regular inflammatory fluoreseer耽microscope．Methodstargeted FITCfluorescenceandrI帕nocl∞aI of contrastmicrobubbleswithFITC anlibodyP—selectin(MBp)andthe嘲llar nu删陀剐x哪ce(MB) were andMB to10 micewithTNF—d或iIImh玛and10 mice constructed．MBpwe兜intravenouslyinjectedwild—type wild—type withouttreaRnent theadhesiontlll／nberandadhesionconditionincremastefie啪uksintwo within5mm any randomly．Then groups wereo】措e蒯andrecorded20t酶nⅦ叽愀nee of by