方法和组分的分析用于增强经皮给药.docVIP

Methods and compostions for enhancing transdermal drug deliveryUS 20090291133 A1 摘要 Methods and compositions for enhancing transdermal delivery of a bioactive agent. The method contains the step of applying to a skin tissue an effective amount of a composition comprising: (a) a drug vehicle; (b) a bioactive agent encapsulated within the drug vehicle; (c) a plurality of proteolytic enzyme molecules conjugated onto the surface of the drug vehicle; and (d) a pharmaceutically acceptable carrier, for a period of time effective to deliver the bioactive agent across the skin tissue at a desired dosage. 图片(3) ? ? ? 权利要求(20) 1. A method for enhancing delivery of a bioactive agent across a skin tissue comprising the step of: applying an effective amount of a composition comprising: a) a drug vehicle; b) a bioactive agent encapsulated within the drug vehicle; c) a plurality of proteolytic enzyme molecules conjugated onto the surface of the drug vehicle; and d) a pharmaceutically acceptable carrier, for a period of time effective to deliver the bioactive agent across the skin tissue at a desired dosage. 2. The method of claim I, wherein the drug vehicle is a liposome. 3. The method of claim 2, wherein the composition further comprises a nanoparticle enclosed in the liposome, wherein the bioactive agent is encapsulated within the nanoparicle within the liposome. 4. The method of claim 3, wherein the nanoparticle is formed from poly(lactic-co-glycolic acid) (PLGA) or hydro-gel. 5. The method of claim 1, wherein the proteolytic enzyme is papain. 6. The method of claim 5, wherein the concentration of papain is less than 93, or less than 50, 40 or 30 μg/ml. 7. The method of claim 1, wherein the proteolytic enzyme is a selected from the group consisting of papain, pancreatin, ficin, bromelain, elastase, pepsin, hyaluronidase, streptokinase, streptodomase, trypsin, chymotrypsin, α-chymotrypsin, α-amylase, deoxyribonucLlease, collagenase, sutilain and any combination thereof. 8. The