维生素C抗辐射损伤作用研究.docVIP

维生素C抗辐射损伤作用研究 时间:2005-01-05 13:31:00?? 来源:食品商务网作者：傅春玲　江伟威　张萍　陈香　朱圣陶单位：苏州大学预防医学系，苏州，215007关键词：维生素C；γ射线；辐射损伤；防护作用；PECMN　　摘要　目的　通过照前给予VitC,观察其对60Co γ射线全身照射小鼠的保护作用。方法　将小鼠随机分为正常对照组、照射对照组、VitC实验组1，2，3(照前连续10d灌胃VitC 15、30、45mg/kg体重)，检测各组小鼠骨髓嗜多染红细胞微核率，并作30d存活率实验。结果　VitC可显著降低2.0Gy γ射线诱发小鼠骨髓细胞微核，可提高受照小鼠30d存活率，延长平均存活时间，保护指数达2.09。结论　VitC对中、高剂量γ射线的损伤具有一定的防护作用。　　中图法分类号　R977.23；R142Protective Effects of Vitamin C against Gamma-ray Induced Wholly Damage and Genetic DamageFu Chunlin, Jiang Weiwei, Zhang Ping, et al(Department of Preventive Medicine,Suzhou University,Suzhou,215007)Abstract　Objective　Protective effects of supplemental vitamin C against 60　Co-gamma-ray induced wholly damage and genetic damage was investigated in mice.Method　Mice were divided into normal control group,irradiation control group and vitamin C experimental group 1,2,3 (which were orally given vitamin C 15,30,45mg/kg.bw for 10 successive days respectively prior to gamma-ray irradiation).Micronuclei in the bone marrow polychromatophilic erythrocytes in each group of mice were examined and the 30 day survival rate of mice following whole-body 5.0 Gy γ irradiation were also determined.Results　Supplemental vitamin C prior to gamma-rays irradiation can significantly decrease bone marrow PECMN rate of mice and increase 30 day survival rate and prolong average survival time.The protection factor is 2.09.Conclusion　Vitamin C has potent protective effects against gamma irradiation induced damage in mice.In certain dose range,vitamin C can absolutely suppress the gamma-rays induced genetic damage in vivo.Key words　VitC;γ-ray;radiation damage;protective effects;PECMN　　维生素C(VitC)又称为抗坏血酸(ascorbic acid)，是人体必需的一种营养素，富含于新鲜的蔬菜、水果中，具有抗氧化作用，可降低DNA的氧化损伤，抑制肿瘤细胞的生长［1～3］。国外研究［4］表明，VitC具有一定的抗辐射氧化作用。由于VitC是一种安全、副作用小、易排泄、天然存在于食品中的成份，因此进一步研究证实其抗辐射作用并开拓其在辐射防护方面的应用具有一定意义。为此，本文通过照前给予VitC，观察其对60Coγ射线全身照射小鼠的保护作用。结果报告于下。1　材料与方法1.1　实验动物与试剂　昆明种小鼠，由苏州大学动物实验中心提供，雌雄各半，体重约20g。抗坏血酸及分析纯购自无锡民丰试剂厂。1.2　动物处理　将40只实验小鼠随机分为正常对照组(正常饲养，不加照射