08麻醉2班回宇峰等海人酸致痫大鼠的海马齿状回分子层微血管构筑的改变.doc

海人酸致痫大鼠的海马齿状回分子层微血管构筑的改变 回宇峰，谭思琪，张媛媛，曹冬冬 指导教师：于胜波 【摘要】 目的 观察海人酸（KA）癫痫模型中海马齿状回分子层微血管构筑的改变。 方法 采用KA癫痫模型，在造模型后7天应用碱性磷酸酶法显示海马脑片的微血管，光镜观察，定量分析。 结果 海马内的微血管成层分布，构筑模式与神经元的构筑模式相一致;KA 组的微血管数目明显多于对照组（P=0.003），血管平均直径无明显变化（P=0.121），血管平均长度明显高于对照组（P=0.000）。 结论 癫痫大鼠海马齿状回分子层微血管构筑的改变是癫痫发病的形态学基础之一。 【关键词】癫痫；海人酸；海马齿状回分子层；微血管；碱性磷酸酶法 The Change of Angioarchitecture of Hippocampus Dentate Gyrus Molecular Layer in Seizure Rats Induced by KA 【Abstract】Objective The quantitative observation of the microvessels of hippocampus Dentate Gyrus Molecular Layer was carried out to explore the change of the angioarchitecture of the hippocampus in the epilepsy rats. Methods The rats induced by KA injection were used as the animal model for epilepsy, and the microvessels of hippocampus were stained by the alkaline phosphatase method at the 7th day after KA injection. The slices of the hippocampus were observed by a light microscope and analyzed quantitatively by NIS Element BR analysis system. Results The microvessels of the hippocampus were arranged in layers consistent with the pattern of the neurons of the hippocampus. The number of microvessels was significantly increased in KA group than in the control group(p=0.003),with the similar average diameter of the microvessels between two groups. However the average length in KA group was longer significantly than in the control group(p=0.000). Conclusions The microvessels changes of the hippocampus Dentate Gyrus Molecular Layer might be one of the morphological bases of epilepsy. 【Key words】Epilepsy; ainic acid; Hippocampus; dentate gyrus molecular layer; microvessels; alkaline phosphatase. 癫痫是由多种病因引起的脑功能障碍综合征，是脑细胞群异常的超同步放电而引起的发作性、突然的、暂时性的脑功能紊乱。海人酸（ainic acid, KA）是脑内兴奋性氨基酸递质谷氨酸的结构类似物。脑内或系统给予惊厥剂量的海人酸，可选择性地激活边缘系统，引起急性癫痫发作。目前，海人酸模型已广泛用于癫痫发作的研究。海马是脑内对缺血最敏感的区域之一。近年来不少学者从不同方面对海马的血管构筑进行了探讨[1-4]。上世纪初Scharrer[5]等就指出海马结构的易损性主要归因于其血液供应的独特方式，并认为许多疾病只要能影响血供，就可能损伤海马内的神经组织。辜清[6]等已在扫描电镜下观察了听源性癫痫大鼠海马血管铸型标本，发现微动脉出现了不同程度和类型的改变。可见，癫痫动物海马内存在微血管病变。在此基础上，本文采用碱性磷酸酶法对KA癫