左旋精氨酸对去势骨质疏松大鼠血清TNF.docVIP

左旋精氨酸对去势骨质疏松大鼠血清TNF- α和NO的影响 杨志军 目的: 观察左旋精氨酸对去势骨质疏松大鼠血清中TNF-α、NO, 探讨抗左旋精氨酸防治骨质疏松症的作用机理。方法: 用左旋精氨酸对去势骨质疏松大鼠进行不同剂量的药物治疗, 并与假手术组、模型组进行比较, 采用双抗体夹心酶联免疫吸附法( ELISA)测定血清中TNF-α, 采用硝酸/还原酶法测定血清中NO含量。结果: 血清中TNF-α、NO, 左旋精氨酸小剂量组与模型组无明显差异( P0.05); 左旋精氨酸大、中剂量组与模型组比较有极显著差异(P0.01),结论: 左旋精氨酸是可以通过降低血清中TNF-α含量, 平衡血清中NO含量, 抑制破骨细胞的活性, 改善骨代谢而达到防治绝经后骨质疏松症的作用。 关键词：左旋精氨酸 骨质疏松 肿瘤坏死因子-α 一氧化氮 Effect of L-arginine on TNF –α and NO in Rat Model of Osteoporosis Abstract: Objective: To observe the effect of L-arginine on TNF –α and NO in rat model of osteoporosis, and investigate its mechanism of action of anti- osteoporosis. Method: Rat models of osteoporosis were treated with L-arginine in different doses. And sham operation group, model group were contrasted with treatment groups. Then TNF-α was detected by double antibody sandwich enzyme linked immunosorbent assay ( ELISA ) , and NO was done by nitrate reductase. Result: There were no differences on TNF-α and NO between low dose treatment group and mode l group (P 0.05). But there was significant difference between large and middle doses of treatment groups and model group (P 0.01). And no difference showed between large and middle doses of treatment groups (P 0.05). Conclusion: L-arginine can prevent and cure postmenopausal osteoporosis by cutting down TNF-α and balancing NO in blood serum, and inhibit the activity of osteoclast. Key words: L-arginine, osteoporosis, tumor necrosis factor-α( TNF-α) , nitrogen monoxidum ( NO) 骨质疏松症( OP)是常见的老年多发性疾病之一, 其发病率高达7%。骨质疏松症的病因病理以及调节机制十分复杂, 至今仍难以阐明, 所以OP 的防治目前尚无理想的方法。研究表明，合理剂量的外源性一氧化氮( NO) 供体能有效的防治绝经后骨质疏松。本实验采用切除大鼠双侧卵巢诱发绝经后骨质疏松症模型, 通过观察左旋精氨酸对去势骨质疏松大鼠血清中TNF-α、NO 含量的影响, 探讨左旋精氨酸的治疗作用。 1 材料和方法 1. 1 材料 1. 1. 1 动物 6月龄健康雌性SD大鼠50只, 体重280-320g, 购自山西医科大学动物实验中心。 1. 1. 2 试剂 L-arginine ( Sigma 批号: A8094 ) , TNF-α和NO试剂盒(上海江莱生物科技有限公司)1. 1. 3 动物分组与造模 大鼠适应性喂养1周, 行卵巢切除手术, 各组大鼠在无菌条件下行10%水合氯醛腹腔注射麻醉。从背部腰椎两侧1cm纵向切口, 切开皮肤, 逐层剪开,钝性分离肌肉, 进入腹腔在肾下极附近找到卵巢, 用4号线结扎并摘除双侧卵巢, 止血、逐层缝合, 用3号手术线缝合切口, 术后75%酒精消毒切口, 伤口涂抹红霉素软膏、肌注青霉素, 预防伤口感染。假