磺胺嘧啶多克隆抗体的制备.pdfVIP

河南农业科学 磺胺嘧啶多克隆抗体的制备 杨继飞，职爱民，刘庆堂，邓瑞广，柴书军，张改平。 (河南省农业科学院河南省动物免疫学重点实验室，河南郑州450002) 电泳试验检测，结果表明偶联成功。用BSA—SD免疫新西兰大白兔，间接EI。ISA测定多克隆抗体 叉反应小于等于2．7％。结果表明，获得了高效价和特异的pAb。 关键词：磺胺嘧啶；多克隆抗体；制备 中图分类号：$859．84文献标识码：A ofthe AntibodiesSulfadiazine PreparationPolyclonal against YANG Ai—rain，I。IU Ji—fei，ZHl Qing—tang， DENG Rui—guang，CHAlShu—jun，ZHANGGai—ping‘ (HcnanProvincial ofAnimal Laboratory Immunology， Key Henan of 450002，China) Sciences。Zhengzhou AcademyAgricultural the antibodiessulfadiazine。theartificial anti— Abstract：To immunizing preparepolyclonal aginst furtheridentified BSA—SDand OVA—SDobtaineddiazotization，and gen coatingantigen by by ultraviolet(UV)andSI)S—PAGE．NewZealandwhiterabbitswereimmunizedwithBSA—SD． Thetitresof antibodiesweredetectedindirectELISAand ELISA．There— polyclonal by blocking suitsshowedthatthe reachedtO1 9 thecrossreactionwithothersulfa IC50 9．1 ng／mI。and drugs waslessthanor to2．7％．ThisindicatedthattheSD antibodiesof titer。sen— equal polyclonal high and hasbeen andmadeit tOestablish ofSD sitivityspecificity generated possible immunoassay residues． Keywords：Sulfadiazine；Polyclonalantibody；Preparation 磺胺嘧啶(sulfadiazine，SI))是磺胺类药物中比障渗人脑脊液。机体对其排泄较慢，有效浓度可维