快速老化模型小鼠SAMP8行为学的增龄性变化.pdfVIP

快速老化模型小鼠SAMP8行为学的增龄性变化 褚芹 于建春 潘建明 于涛 韩景献 （指导老师） [摘要] 目的对SAMP8 老化征象和学习记忆能力的增龄性变化进行比较系统的研 究，为利用该模型进行其他研究提供实验依据。方法 选用 2 、4 、6、8、10、12 月龄SAMP8，以同龄正常老化的SAMR1 为对照，应用老化度评分和Morris 水迷 宫测试SAMP8 的老化度和学习记忆能力的增龄性变化。结果 SAMP8 在4 月龄出 现明显的老化体征，并在6 月龄开始表现出空间学习记忆障碍。结论 SAMP8 在成 熟期（4-6 月）过后发生了快速老化，证实SAMP8 是研究衰老和老年性痴呆的理想 模型。 [关键词] 快速老化模型小白鼠 （SAM）;SAMP8;老化;Morris水迷宫; 学习记忆 能力 Age-related Changes in behavior of Senescence Accelerated Mouse SAMP8 Chu Qin , YU Jianchun , Pan Jianming, Yu Tao, Han Jingxian ( The First Hospital affiliated to Tianjin University of TCM Tianjin China 300193) [Abstract] Objective To observe the age-related changes in aging sign and symptoms, and learning and memory of senescence accelerated mouse SAMP8. Method selecting Senescence Accelerated Mouse SAMP8 and normal control SAMR1 at six ages from 2th month to 12th month, age-related changes in aging and learning and memory were studied by “the scores of aging”and Water Morrris Maze. Results The SAMP8 appeared obvious aging sign and symptoms at 4-month old. Compared to SAMR1 control group, SAMP8 mouse showed significant impairments on learning and memory in hidden platform trial at age of 8th month, 10th month and 12th month. In probe trial and reversal trial, SAMP8 began to show significant impairments on learning and memory at age of 6month. Conclusion The results showed that SAMP8 began to accelerate senescense after mature period (4- 6month) and appeared learning and memory disorder at 6-8 months old. It manifested that SAMP8 was an ideal model for investigating the mechanism of aging and senile dementia. 基金项目：教育部高等学校博士学科点专项科研基金 （20060063006） 作者单位： 300193天津 天津中医药大学第一附属医院基础研究部 （褚芹、于建春、于涛、韩景献），天津中 医药大学解剖教研室 （潘建明 ） 。联系电话：022Email:cq0314@ 1