粘细菌BD105产生的抑瘤活性物质的分离制备.pdfVIP

ofAnhui 安徽农业科学。Journal A蜊．Sci．2010，38(1I)：5531—5532。5543 责任编辑马树梅责任校对傅真治 粘细菌BDl05产生的抑瘤活性物质的分离制备 张耕凇，张利平’ (河北大学生命科学学院，河北省微生物多样性研究与应用重点实验室，河北保定071002) 摘要从河北省微生物多样性研究与重点实验室保存的具有抑瘤活性的粘细菌BDl05菌株中分离纯化了抑制人宫颈癌细胞的活性物 质。应用分析型高效液相色谱对BDl05菌株的发酵产物甲醇粗提液进行梯度洗脱，确定混合发酵产物的最适分离条件。再运用制备型 高效液相色谱对不同组分进行分离，大量发酵后制备得到组分I(浅黄色粉末状化舍物)。应用MTT法对得到的活性组分进行体外抗癌 细胞活性检测，发现组分I对人宫颈癌细胞最高抑制活性达91．785％，体内动物实验也显示出高效低毒的生物活性。 关键词粘细茵；分离制备；-E癌活性 中图分类号Q93文献标识码A 文章编号0517—66ll(2010)1l一05531—02 TheIsolationand ofAnti-tumorActive Produced BDl05 Preparation Agent byMyxobacteria et ofLife LabofMicrobial and of ZHANGLin-song Science，Hebei Research HebeiProv．， ai(CoUege University，Key Diversity Application 071002) Baoding．Hebei AbstractAnactivesthstaneeofanti—humancervicalcsncerwas and砌edin strainBDl05whiekstoredin labof separated myxobaetefia key mierobial researchand ofHebei fermentationofthisstrainwasextractedmethanoland diversity aDplicationprovince．The products by gradienfly HP【Cinordertoconfirmthe conditionsofdifferent these were elutedbyanalytical optimumseparation components．Thencomponentsseparated HPLCandtheactivesubstanceofanti—tiM／lotw鹧testedwithMTr the of com- bypreparative assay．Underguidancebiologicalactivitytracking．a named IWaS whichwasflavescens its tohumancervicalcancercellsachieyed pound Componentgot powder．andhishestinhibitoryactivity onanimalsalsoshowedthatthis hada withlowtoxin． 91．785％．Experimentation compoundhighefliciency words and Key Myxobaeteria；Anti-tumoractivity；Isolationpreparation mm×4．6