荧光小分子pH探针的最新研究进展.pdfVIP

广 东 化 工 2010 年 第12 期 · 56 · 第37 卷 总第212 期 荧光小分子pH探针的最新研究进展 马京 (山东师范大学 化学化工与材料科学学院，山东 济南 250014) + + [摘 要]细胞内的H 在生理及病理过程中发挥着至关重要的作用。因此，研究细胞内的H 具有重要的理论和实践意义。荧光法由于其非破 坏性，高的灵敏度和选择性，以及广泛可用的荧光染料在测定pH 时比其他方法更具优越性。文章以不同的荧光团分类：香豆素、荧光素、罗丹 明、BODIPY、花菁、双光子染料等，按pKa 从小到大的顺序排列，综述了近十年来，荧光小分子pH 探针的研究进展。 [关键词]荧光；pH ；小分子探针；综述 [中图分类号]TQ [文献标识码]A [文章编号]1007-1865(2010)12-0056-02 The Latest Researching Development of Fluorescent Small- molecule pH Probes Ma Jing (College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China) Abstract: Intracellular hydrogen ion plays many critical roles in physiological and pathological process. Studying hydrogen ion changes inside living cells is significantly meaningful in theory and practice. Fluorescence methods offer significant advantages over other methods due to its generally nondestructive character, high sensitivity and selectivity, and the wide range of indicator dyes available. The paper was based on the different categories of the fluorophore: coumarin, fluorescein, rhodamine, BODIPY, cyanine, two-photon dye and others. It described the researching development of the fluorescent small-molecule pH probes in the last decade, and ordered by the pKa from small to large. Keywords: fluorescence ；pH ；small-molecule probes ；review 细胞内pH在细胞的许多生理及病理过程中起着关键性的 Burgess等[14]将BODIPY与两分子荧光素相连，合成化合 [1] [2] [2b] 物69 ，检测细胞中的pH( 响应范围4.0~6.5 ，7.0~7.9) 。探针利用 作用 ，例如，细胞的增殖和凋亡 、细胞内吞