分子与基因工程实验技术.docVIP

Appendix D: Experimental Techniques 附录D: 实 验 技 术 Elucidating the molecular basis of life is no easy task. Our current understanding of complex processes like replication, transcription or translation has required thousands of scientists laboring for decades. Yet, considering the tiny scale on which molecular biologists work, the amount known is admirable. This understanding is due to a powerful set of techniques, tools that allow our immense hands to cut and past molecules, and our blurry eyes to peer inside cells. In this chapter we review the most important of these techniques used