Determination of thiol compounds in rat striatum microdialysate by HPLC with a nanosized CoHCFmodif.pdfVIP

Anal Bioanal Chem (2004) 380: 742–750 DOI 10.1007/s00216-004-2838-0 ORIGINAL PAPER Meichuan Liu Æ Ping Li Æ Yuxiao Cheng Æ Yuezhong Xian Chenglin Zhang Æ Litong Jin Determination of thiol compounds in rat striatum microdialysate by HPLC with a nanosized CoHCF-modified electrode Received: 10 June 2004 / Revised: 12 August 2004 / Accepted: 24 August 2004 / Published online: 6 November 2004 Springer-Verlag 2004 Abstract A cobalt hexacyanoferrate (CoHCF) nano- xenobiotics, which are thought to contribute to the particle (size ca. 60 nm) chemically modified electrode development of many common diseases including can- (CME) was fabricated and the electrochemical behavior cer, heart attack, stroke, arthritis and Parkinson’s Dis- of thiols at this nanosized CoHCF CME was studied. In ease [2–4]. Other functions include maintenance of thiol comparison with a bare glassy carbon (GC) electrode redox potential in cells by reducing the thiol groups of and with a general CoHCF CME which was electrode- proteins, transport and storage of cysteine, and as a posited in the traditional manner, the present nanosized cofactor in some isomerization reactions [5, 6]. L-Cys is CoHCF CME efficiently performed electrocatalytic an excitable amino acid and a neurotransmitter in the oxidation for glutathione (GSH) and L-Cysteine (L-Cys) brain [7, 8]. It plays an important role in communication with relatively high sensitivity, outstanding stability, and among immune-system cells [9] and is also critical to the long-life. Combined with high-performance liquid metabolism of a number of essential biochemicals, for chromatography (HPLC), the nanosized CoHCF CME example coenzyme A, heparin, biotin, lipoid acid, and was used for electrochemical determination (