免疫状态局部和全身的炎症反应与急性重症胰腺炎.pdfVIP

Scandinavian Journal of Gastroenterology, 2006; 41: 472/480 ORIGINAL ARTICLE Immune status and inflammatory response differ locally and systemically in severe acute pancreatitis 1 1 2 2 CHANGBIN SHI , XIA ZHAO , ANNA LAGERGREN , MIKAEL SIGVARDSSON , 3 XIANGDONG WANG3 ROLAND ANDERSSON1 1 / 7 0 / 1 2 0 Department of Surgery, Lund University Hospital, Lund, Sweden, Department of Hematopoietic Stem Cell Biology, 1 n Lund Strategic Center for Stem Cell Biology and Cell Therapy, Lund, Sweden, and 3Department of Respiratory Medicine, o e n Zhongshan Hospital, Fudan University, Shanghai, China r u o b l e M f o y Abstract t i s r Objective. Acute pancreatitis (AP) is an inflammatory disorder that develops a complex cascade of immunological events. e v i The local and systemic immune status and inflammatory response might contribute to the understanding of underlying n U pathophysiological mechanisms and potential treatment. Material and methods. Severe AP was induced by intraductal y b perfusion of 5% sodium taurodeoxycholate in rats. mRNA expression of cytokines and chemokines was determined by m y. reverse transcriptase-polymerase chain reaction (RT-PCR) and NF-kB activation was assessed by electrophoretic mobility o l c n e. o shift assay in fresh pancreatic acini and circulating monocytes 1, 3, 6 or 9 h after sham operation, induction of AP or r e a s c u N-acetylcysteine