原料奶中金黄色葡萄球菌分离鉴定及产毒菌株的快速检测.pdfVIP

2011, 39( 5) : 140 ~ 14 3 G uizhou A gr icul ural Sciences [ ] 1001- 3601( 2011) 05- 0 314- 0 140- 04 原料奶中金黄色葡萄球菌分离鉴定及产毒菌株的快速检测 * 覃艳华, 谢 和 , 胡 萍, 李荔枝 ( , 550025) [ ] 为了解原料奶中产毒型金黄色葡萄球菌的污染情况, 采用传统分类学鉴定 法与P CR 鉴定 法结合, 对原料奶中金黄色葡萄球菌进行分离鉴定, 并运用全自动荧光免疫分析仪检测了分离菌株的产毒 情况结果表明, 分离鉴定的70 株金黄色葡萄球菌中, min-i V IDA S 仪分析为阳性的有 9 株, 产毒型金黄色 葡萄球菌的检出率为12. 86% , 金黄色葡萄球菌为原料奶的主要污染菌之一针对金黄色葡萄球菌耐热核 酸酶基因设计的引物进行的PCR 鉴定 法, 与传统分类学 法的符合率为 100% , 并且具有较高的特异性 及敏感性 [ ] 金黄色葡萄球菌; 肠毒素; 分离鉴定; P CR; 全自动荧光免疫分析仪 [ ] S811. 6 [ ] A Isolat ion and Identification of S a phylococcus aureus in Raw Milk and Rapid Detection of Toxigenic Strains * QIN Y an- hua, X IE H e , H U Ping, LI L-i zhi ( Colleg e of Lif e Science, Guiz hou Univers ity , Guiy a ng, Guiz hou 550025, China) Abstract: S rains of St ap hy l ococcus aure us w ere isola ed and iden if ied fro m raw milk by radi ional axo nom ic iden ifica ion and pol ymerase chain reac ion ( P CR ) , and heir ox in producing abili y w as de ec ed by m in-i V IDA S o s udy he S. a ur eus con amina ion in r aw milk . T he resul s show ed ha S. aure us w as he main co n amina ion bac eria, 9 s rains of hem w ere posi ive w hich de ec ed by min-i V IDA S, and he de ec io n ra e of ox igenic s rains w as 12. 86% in he 70 s rains of S. a ur eus isola ed. T he P CR designed arg e ing he prim er of hermo nuclease( n uc) gene of S. a ur eus had co incidence r a e of 100 % w i h radi io nal ax onomic iden if ica io n, and i had hig her specifici y and sensi ivi y. Key words: Stap hy lococ cus aur eus ; en er