反硝化菌功能基因及其分子生态学研究进展.pdfVIP

微生物学通报 APR 20, 2011, 38(4): 583−590 Microbiology China © 2011 by Institute of Microbiology, CAS tongbao@ 专论与综述 反硝化菌功能基因及其分子生态学研究进展 郭丽芸 时飞 杨柳燕* (污染控制与资源化研究国家重点实验室 南京大学环境学院 江苏 南京 210093) 摘 要: 由微生物推动的反硝化作用是地球氮素循环的重要分支, 尽管已被发现广泛存在于细 菌、真菌和古生菌中, 其功能基因的研究仍仅限于很少几个物种。现代分子生物学的发展为研究 环境微生物提供了行之有效的方法, 以反硝化功能基因作为分子标记的分子生态学研究迅猛发 展。综述近年来国内外微生物反硝化功能基因研究及以其为标记的分子生态学研究进展。 关键词: 反硝化细菌, 功能基因, 分子生态学 Advances in functional genes and molecular ecology in denitrifiers * GUO Li-Yun SHI Fei YANG Liu-Yan (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210093, China) Abstract: Denitrification, a microbial process, is a major branch of the geography nitrogen cycle. Al- though the denitrifying ability has been widely found in bacteria, fungi and Archaea, the genes encoding the denitrifying reductases have been studied in few species. Modern molecular biology techniques provide effective methods to study the microbial ecosystem, and important knowledge has been accu- mulated on using functional genes for molecular techniques in the case of denitrification. Recent inves- tigations on functional genes and molecular ecology in denitrifiers have been summarized. Keywords: Denitrifiers, Functional genes, Molecular ecology 反硝化作用是氮素循环中的一个重要生物过 应气体, 如 NO 和 N2O