多年生簇毛麦a醇溶蛋白基因的分离与序列分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2008, 34(6): 1097−1103 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2008.01097 多年生簇毛麦α-醇溶蛋白基因的分离与序列分析 * * 李光蓉 任正隆 刘 成 周建平 杨足君 (, 610054) : (Dasypyrum breviaristatum)DNA , PCR , 999~1 018 bp 7 (GenBank EU186102–EU186108), 283~290 , α-, α-7 (celiac disease), 5 C Glia-α-2 α-, α-, AB D α- AS-V-gli-F AS-V-gli-R, PCR , AS-V-gli-F AS-V-gli-R - , , AS-V-gli-F AS-V-gli-R α- : ; α-; ; PCR Isolation and Sequence Analysis of α-gliadin Genes from Dasypyrum breviaristatum * * LI Guang-Rong, REN Zheng-Long , LIU Cheng, ZHOU Jian-Ping, and YANG Zu-Jun (School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China) Abstract: PCR was carried out on the genomic DNA from Dasypyrum breviaristatum using the conserved primers specific forα-gliadin genes. The PCR products were cloned and sequenced. Total 7 sequences were obtained with length of 999 to 1 018 bp containing coding regions from 283 to 290 amino acids and their GenBank accession number are EU186102 to EU186108 respec- tively. BLAST search showed that these sequences belong to α-gliadin super-family. Searching for 4 different T cell stimulatory epitopes for celiac disease patient, it is found that 5 of 7 sequences contained the Glia-α-2 epitopes at the C terminal region. The phylogenetic trees indicated