微生物壳聚糖酶的研究概况.pdfVIP

浙江大学学报( ) 30( 2) : 229236, 2004 Journal of Zhej iang University (Agric. Life Sci. ) : 1008-9209(2004) 02-0229-08 戴 芸, 朱旭芬 ( , 310012) : 壳聚糖是自然界中最丰富的多糖之一. 壳聚糖酶能够降解壳聚糖生成壳寡糖, 壳寡糖具有多种 生 学特性, 用途广泛. 壳聚糖酶主要分布在微生 和 一些植 中, 理化性质较稳定. 根据其底 特异性 和断裂键的专 一性可分为 Ⅰ、Ⅱ、Ⅲ三类. 根据已测序壳聚糖酶的氨基酸序列相似性, 壳聚糖酶分别属 于糖苷水解酶46 号、75 号和80 号. 目前, 只有2 种壳聚糖酶的3 级结构被测定. : 壳聚糖; 壳聚糖酶; 糖苷水解酶; 氨基酸序列 : Q556　　　: A , - ( . , , 310012, ) DAI Yu ZHU Xu fe Dep t of BioSciences Zhej iang University H angzhou China Progress of research on microbiological chitosanases. Jour al of Zhejia g U iversit y (Agric. Life Sci.) , 2004, 30( 2) : 229-236 : . -1, 4- Abstract Chit osa is o e of t he most abu da t polysaccharides i ature It co sists of li ked D-glucosami e ( GlcN) a d N-acet yl--Dglucosam i e ( GlcNAc) residues. Chitosa ases catalyze the hy- drolytic breaki g of its glucosidic bo d a d produce chitosa , which are widely applied for various pur- poses i medici e, agricult ure, food a d i dustry . Chitosa ases fou d i microorga isms a d som e . pla ts have stable physical a d chemical properties They are classified i to three subclasses based o t he substrate specificit y a d the cleavage positio s of the partially acetylated chit osa . Accordi g to t he ami o acid alig me t , most seque ced chit osa ases belo g to three-glycoside hydrolase f amilies desig- ated as umber 46, 75 a d 80. T he three dime sio al struct ures have bee determi ed o ly for t he . 174 - 1. chit osa ases from Strep tomycse sp N a d Bacillus circulans M H K