低压缺氧对兔动脉粥样硬化斑块中胶原含量的影响(论文资料).pdfVIP

检验医学与临床2010 年10 月第7 卷第20 期 Lab Med Clin, October 2010, V ol. 7, No. 20 % 2183 % 1 1 2 1 1 张司兰 , 徐立群 , 韩 威 , 陈世伟 , 谢 凤 ( 1.重庆市璧山县人民医院心血管内科 402760; 2. 成都医学院医学 验系实验中心, 成都 610083) 观察低压缺氧对兔动脉粥样硬化斑块胶原含量的影响, 并对其机制进行初步探讨 日 本大耳兔随机分为常氧对照组和低压缺氧组两组, 高胆固醇饮食 12 周建立兔动脉粥样硬化(atherosclerosis,AS) 病 变模型后, 取主动脉内膜组织分别采用Woessner 法western blot 法和实时荧光定量逆转录聚合酶链反应( RT CR) 法 测斑块组织中胶原含量人核转录因子( NFB) 蛋白肿瘤坏死因子(T NF) 白细胞介素 6 逆转核糖核 酸(IL6 mRNA) 表达 低压缺氧能明显降低斑块中胶原蛋白含量, 提高 NFB 蛋白TNF和IL6 mRNA 表达(P 0.05) 低压缺氧能明显降低斑块中胶原蛋白含量, 其机制可能与促进炎性反应, 升高NFB 蛋 白TNF及IL6 mRNA 表达有关 动脉粥样硬化; NFB; 肿瘤坏死因子; 白细胞介素6 DOI: 10. 3969/ j. issn. 2010. 20.004 :R543.5 : A :2010) 1 1 Effects of hypobaric hypoxia on content of collagen in rabbit atherosclerotic plaques ZHAN G Silan ,X U Liqun , 2 1 1 HAN Wei , CH EN Shiwei , XI E Feng . 1. Dep art ent of Cardiology , Bishan Peop le! s H osp ital, Bisan Chongqing 402760, China; 2. Exp eri ental Center, dep art ent of Medical Laboratory , Chengdu Medical College, Chengdu, Sichuan 610083, China Abstract Objective T o study the effects of hypobaric hypoxia on collagen content in atherosclerotic plaques of rabbit models and to init ially investigate its mechanism. Methods Rabbit atherosclerot ic models w ere set up by highcholesterol diet. T he contents of NFB and collagen in plaque w ere det ected by Western blot and w oessner met hod, respectively.Expression levels of T NF, IL6 mRNA w ere determ ined w ith realtime CR.Results In hy pobaric hypoxia group, collagen in atherosclerotic plaques w as decreased markedly, TNF, IL6 mRNA and NFB protein found in atherosclerotic plaques w ere elevat ed. Conclusion Hypobaric hypoxia could decrease the collagen cont ent in rabbi