《EaIspF1 Essential Enzyme in Isoprenoid Biosynthesis from Eupatorium adenophorum Reveals a Novel Role in Light Acclimation》.pdfVIP

Journal of Integrative Agriculture 2014, 13(5): 1024-1035 May 2014 RESEARCH ARTICLE EaIspF1, Essential Enzyme in Isoprenoid Biosynthesis from Eupatorium adenophorum Reveals a Novel Role in Light Acclimation , ZHANG Sheng-rui, JIANG Xue, WANG Ping, WU Di, WANG Qing-hua and HOU Yu-xia College of Science, China Agricultural University, Beijing 100193, P .R .China Abstract Isoprenoids are a functionally and structurally diverse class of natural organic chemicals. The universal precursors of all isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate are synthesized through the mevalonate and 2C-methyl- D-erythritol 4-phosphate (MEP) pathways, respectively. Many isoprenoids produced through the MEP pathway play an important role in plant acclimation to different light environments. Eupatorium adenophorum, an invasive weed in China, presents a remarkable capacity to acclimate to various light environments, which constitutes its solid foundation of being a successful invasive species. Thus we aimed at gaining a deeper insight into the regulation of MEP pathway in E . adenophorum to further understand the invasive mechanism. 2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF or MCS) is an essential enzyme in the MEP pathway. In this paper, a novel IspF gene was cloned and characterized from E . adenophorum. Tissue-specific expression assays revealed a higher expression of EaIspF1 in leaves than in stems and roots. The expression of EaIspF1 was responsive to different light conditions. Some up-regulation of EaIspF1 expression was also found after the treatments with signal compounds and after wounding stress. Interestingly, the over-expression of EaIspF1 in Arabidopsis led to increase carotenoids contents,