《Effect of Daidzein on Ileum Microlfora Biodiversity in HyLine Variety Brown Layers》.pdfVIP

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《Effect of Daidzein on Ileum Microlfora Biodiversity in HyLine Variety Brown Layers》.pdf

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December 2014 Vol 21 No 4 31-36 . . ScienceDirect Journal of Northeast Agricultural University (English Edition) Available online at Effect of Daidzein on Ileum Microflora Biodiversity in Hy-Line Variety Brown Layers * Ji Guo-zhen, and Wang Li College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China Abstract: Daidzein is always added into poultry feed to make the production performance and immunity of poultry better. In this study, a total of 600 40-week-old Hy-Line variety brown layers were randomized into five groups and fed with a corn-soybean-mixed -1 • basal diet supplement with 0, 10, 50, 100, and 500 mg kg daidzein, respectively. Then, two PCR-based typing methods (RAPD-PCR and ERIC-PCR) were combined to analyze the ileum content and explore the changes of ileum microflora biodiversity. The results of -1 -1 • • RAPD-PCR and ERIC-PCR showed that bands under 10 mg kg and 50 mg kg were the most, and their similarity was the largest. -1 • Bands under 500 mg kg were the least and similarity with other groups was the minimum. Ileum microflora biodiversity under 10 -1 -1 -1 -1 •