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rlink.com ChinJCQncerRes 2J3
www.spnnge 24(3):213—219,2012
i—嘲隧醚囊獬
EffectofTrastuzumabonNotch一1 inBreast
Pathway
Signaling
CancerSK.BR3Cells
+
MingHan, Hua—yuDeng,RongJiang
StemCe||ondTlssue
DeportmentofPothophysiotogy,LQborotoryfor EngjneerIng.ChongqlngⅣ|edIca|University,
480016,ChinQ
Chongqjng
10.1007,sll670—012一0213-9
and Ber¨n
@ChineseAnt卜CancerAssocIation
Springer—VerlagHeideIbe唱2012
ABSTRACT
thee仟ectsandmechanismsoftrastuzumabonNotch一1 inbreastcancer
0biective:Toinvestigate pathway cells,
the 0fI、Jotch一1 intrastuzumabresistance.
recOgnizingsignificance signaIin只pathwaV
andWestem were to the of
Methods:Immunocytochemistrvstaining bIottingempIoyedlustifye×pression
Notch一1 in SK—BR3ceI|sand breastcancerMDA—MB一231ce|ls.
proteinHER2—0verexpressingHER2一nOn一0verexpressing
Western andreverse usedtodetecttheactivatedNotch一1andNotch一1
blottjng transcriptionPCR(RT—PCR)were target
FfES.JmRNA afterSK.BR3ce|Isweretreatedwithtrastuzumab.DoubleimmunofIuorescence
gene expression staining
and wereusedto the off、Jotch一1andHER2
co.inlnlunoprecipitationanaIVzereIationship proteins.
Results:TheIevelOfNotch一1nuclearIocaIIzationandactivatedI、Jotch一1 in ce¨s
proteins
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