《Effect of type2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes》.pdfVIP

NEURAL REGENERATION RESEARCH January 2014, Volume 9, Issue 2 Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes 1 1 1 2 1 1 2 Chunling Fan , Hui Wang , Dan Chen , Xiaoxin Cheng , Kun Xiong , Xuegang Luo , Qilin Cao 1 Department of Human Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, Hunan Province, China 2 The Vivian L Smith Department of Neurosurgery, UT Medical School at Houston, Houston, TX, USA Abstract Corresponding author: The role of type-2 astrocytes in the repair of central nervous system injury remains poorly un- Xuegang Luo, Department of Human derstood. In this study, using a relatively simple culture condition in vitro, type-2 astrocytes, Anatomy and Neurobiology, Xiangya differentiated from oligodendrocyte precursor cells by induction with bone morphogenetic pro- School of Medicine, Central South tein-4, were co-cultured with dorsal root ganglion neurons. We examined the effects of type-2 University, Changsha 410013, Hunan astrocytes differentiated from oligodendrocyte precursor cells on the survival and growth of Province, China, xgluo@. Qilin dorsal root ganglion neurons. Results demonstrated that the number of dorsal root ganglion Cao, The Vivian L Smith Department neurons was higher following co-culture of oligodendrocyte precursor cells and type-2 astrocytes of Neurosurgery, UT Medical School than when cultured alone, but lower than that of neurons co-cultured with type-1 astrocytes. at Houston, Houston, TX 77030, USA, The length of the longest process and the length of all processes of a single neuron were shor