免疫损伤-高脂喂养法建立兔动脉粥样硬化模型评价.pdfVIP

·264· doi：10．3969／j．issn．1008—0341．2009．03。027 免疫损伤一高脂喂养法建立兔动脉粥样硬化模型评价 邵晓军。王海萍，张晨 (青岛大学医学院附属医院神经内科，山东青岛266003) [摘要] 目的探讨建立兔动脉粥样硬化模型的方法。方法成年健康新西兰兔lo只，随机分为两组，对照 组兔常规饲养；模型组兔应用小牛血清清蛋白免疫损伤，高脂饮食喂养，两组均饲养8周。经耳缘静脉取血检测胆 固醇、三酰甘油、高密度脂蛋白、低密度脂蛋白及血浆溶血磷脂酸水平；取颈内动脉，应用苏木精一伊红(HE)染色及 明显的动脉粥样硬化斑块，Emmprin和TIMP一1未见明显表达。结论免疫损伤一高脂喂养法不易于短期内建立兔 动脉粥样硬化模型。 [关键词]动脉硬化；模型，动物；兔 [中图分类号]R543[文献标识码]A[文章编号]1008—0341(2009)03—0264—03 EVALUATIONoFTHEMETHODoF A ESTABLISHINGRABBITMODELoFATHEROSCLERoSISTHROUGHIMMUNOLESION ASSOCIATEDWITHHYPERCHOLESTEROLDIET SHAOXlACpJUN。WANG of Affiliated of Medical Neurology，TheHospitalQingdaoUniversityCollege，Qingdao266003，China) To themethodof arabbitmodelofatherosclerosis．MethodsTenadult [ABSTRACT]Objectiveexplore establishing New healthyZealandrabbitsweredividedintocontrolandmodel rabbitsincontrol werefed andthe groups．The group routinely modelrabbitswerefedwith dietafter albumin，allwerefedfor weeks．Be— hypercholesterolimmunologicinjurybysolcoseryl eight foreand weeksafterthe with eight feedinghypercholesteroldiet，the indexesweredetected：bloodtotalcholesterol following (TC)，triglyceride(TG)thighdensity cholesterol(HDL-C)，low lipoprotein densitylipoprotein(LDL-C)andlysophosphatidic acid(LPA)．HEwasusedto theatheroselerotielesionand WaSusedto staining verify immunohistoehemistyextracellular verify matrix inhibitor1 internalcarotid metaIloprotei