3种方法提取大肠杆菌E.coli脂多糖的比较.pdf

中国麈学通报 2010。26(21)：12—15 Chinese ScienceBulletin Agricultural 3种方法提取大肠杆菌E砌脂多糖的比较 康洁 (商丘师范学院生命科学系，河南商丘476000) 摘要：寻找大肠杆菌EcD矗脂多糖的最佳提取方法。分别用改良热酚法、超声波处理法、煮沸法从大肠 杆菌细胞壁中提取脂多糖，经纯化后分别在721分光光度仪上测其吸光值；再用3种方法提取的脂多糖 分别免疫草鱼，计数法测受试草鱼血液中白细胞的数量，愈创木酚法测受试鱼不同组织中过氧化氢酶的 活力(用OD值表示1。改良热酚法、超声波处理法、煮沸法提取的脂多糖在稀释15倍时，其吸光值分别是 杆菌脂多糖浓度高、免疫活性强，是相对比较好的一种提取方法。 关键词：脂多糖；热酚提取法；超声波提取法；煮沸提取法；过氧化氢酶 中图分类号：$963．1 文献标志码：A 论文编号：2010—1695 to ofThreeDifferentMethodsExtractE．coli Comparision Lipopolysaccharide Jie Kang Normal Henan (Departmentof啦Science,ShangqiuCollege，Shangqiu476000) Abstract：Toand the ofthe ofE．eoli．The study optimizeextractingtechnologylipopolysaccharideimproved hot method，ultrasonic wereusedtoextractthe fromtheE．colicell phenol treatment，boiling lipopolysaccharide the ofthree were methods anddetectedthe valuewith721 wall，andproducts purified absorption thenwere the ofwhite in thenumber differentlyinjectedgrasscarp，tested spectrophotometer,and corpuscles bloodandcatalase indifferentthetissueof with shownwith grasscarp activity grasscarp guaiacolmethod(data OD extractedwiththe hot and methodat value)．Thelipopolysaccharide improvedphenol，ultrasonicboiling diluted15-foldswas detectedOD and