Activity of a maize ubiquitin promoter in transgenic rice外文翻译.pdfVIP

Plant Molecular Biology 23: 567-581, 1993. © 1993 Kluwer Academic Publishers. Printed in Belgium. 567 Activity of a maize ubiquitin promoter in transgenic rice Maria-Jesfis Cornejo 1., Diane Luth 2, Kathleen M. Blankenship 3, Olin D. Anderson and Ann E. Blechl Western Regional Research Center,Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA (* authorfor correspondence); current addresses: ~ Departamento de Biologia Vegetal, Facultad de Farmacia, Universidad de Valencia, Avda. VicenteAndres Estelles s/n, 46100 Burjassot, Valencia, Spain; 2Department of Fruit Crops, University of Florida, Gainesville, FL 32611, USA; 3 Neurogenetics Laboratory, University of California, San Francisco, CA 94143, USA Received 9 April 1993; accepted in revised form 28 July 1993 Key words: GUS expression, heat shock, Oryza sativa L., rice transformation, ubiquitin Abstract We have used the maize ubiquitin 1 promoter, first exon and first intron (UBI) for rice (Oryza sativa L. cv. Taipei 309) transformation experiments and studied its expression in transgenic calli and plants. UBI directed significantly higher levels of transient gene expression than other promoter/intron combinations used for rice transformation. We exploited these high levels of expression to identify stable transformants obtained from callus-derived protoplasts co-transfected with two chimeric genes. The genes consisted of UBI fused to the coding regions of the uidA and bar marker genes (UBI:GUS and UBI:BAR). UBI:GUS expression increased in response to thermal stress in both transfected protoplasts and transgenic rice calli.