《Major physiological adjustments in freezingtolerant grey tiger longicorn beetle Xylotrechus rusticus during overwintering period》.pdfVIP

Journal of Forestry Research (2014) 25(3): 653−659 DOI 10.1007/s11676-014-0504-8 ORIGINAL PAPER Major physiological adjustments in freezing-tolerant grey tiger longi- corn beetle (Xylotrechus rusticus) during overwintering period Jue-wen Li • Juan Shi • Yu Xue • Hong-bo Mao • You-qing Luo Received: 2013-10-18; Accepted: 2013-12-10 © Northeast Forestry University and Springer-Verlag Berlin Heidelberg 2014 1 Abstract: Xylotrechus rusticus (Linnaeus) is one of the most destruc- the bodies of larvae changed significantly during winter, although both tive woodborers in northeast China; it damages poplar and is listed as a had no correlations with the SCP. domestic forestry quarantine pest. Overwintering larvae were collected Keywords: cold hardiness; cryoprotectant; freezing tolerant; physiologi- during October 2012 and March 2013 in Harbin, China, to quantify indi- cal variation; Xylotrechus rusticus . cators related to the insect’s overwintering strategy and the major cryo- protectants. The supercooling points (SCPs), which ranged from –14.7°C to –2.9°C, were higher than the lethal temperatures of LT50 (–33.64°C) Introduction and LT99 (–40.17°C) after 24 h exposure. , also the minimum mean daily temperature (–24.5°C) and mean monthly temperature (–18.0°C) at the In temperate regions, insects face a great challenge surving in sampling site in January during 2008–2012. Thus, X. rusticus is a typical extremely low temperatures (Lee 1989; Koš