A LOV2 Domain-Based Optogenetic Tool.pdfVIP

Chemistry Biology Resource A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function Christian Renicke,1 Daniel Schuster,1 Svetlana Usherenko,1 Lars-Oliver Essen,2 and Christof Taxis1,* 1 ¨ Department of Biology/Genetics, Philipps-Universitat Marburg, Karl-von-Frisch-Strasse 8, 35043 Marburg, Germany 2 ¨ Department of Chemistry and Biomedical Research Center, Philipps-Universitat Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany *Correspondence: taxis@biologie.uni-marburg.de /10.1016/j.chembiol.2013.03.005 SUMMARY coded proteins to control protein activity by light is generally referred to as optogenetics (Christie et al., 2012). So far, several Light perception is indispensable for plants to light-induced expression systems have been developed that respond adequately to external cues and is linked allow regulation of protein abundance (Kennedy et al., 2010; to proteolysis of key transcriptional regulators. To Levskaya et al., 2005; Shimizu-Sato et al., 2002; Sorokina provide synthetic light control of protein stability, et al., 2009), whereas light-dependent manipulation of protein we developed a generic photosensitive degron stability has been largely neglected. The only available method (psd) module combining the light-reactive LOV2 involves intracellular formation of reactive oxygen species, which may induce unwanted side effects (Buli