兔骨髓间充质干细胞对喉返神经损伤后修复研究.pdfVIP

Abstract 一 objeetive： 1．Toestablish锄efficientand for and rabbitbonemarrow culturing simplesystemisolating stem mesenchymalcells(BMSCs)． 2．Toestablishasuccessfulanimalmodelof recurrent injuring laryngealnerve㈣，and themodel． theBMSCsintothe observetheeffecton nerve，then transplant laryngeal Methods： withwholebonemarrowadherentculturemethod． 1．ToisolateandculturerabbitBMSCs thecells舶mcell andfunctional andcharacteristicsof Identify morphology aspects．Themorphology culturedcellswereobserved contrast andadherenceratechiveof byphase microscope．Thegrowth inducedintoosteoblastsand inductor． cellsat 3weredrawn．TheBMSCswere adipocytesby passage halfofthenerveunderthe 2．To theleftofRLNandcutoff expose microscope，thentransplant theBMSCsintothemodel measurethe nerveaction immediately．Tocompound before and4weeksafter the statistical damage，mjuryimmediately injury．Tocompareanalysis make slicestoobserved． and significancepathological Results： culturedBMSCsstarted to 24hoursafter and 1．Primary adheringplates seedinglong wereobserved6 later．Theadherenceratesindifferentculturehour and12 spindle．shaped clays 2，4，8