《Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis》.pdf

A R T I C L E S y Genome sequence of the lignocellulose-bioconverting g o l o n and xylose-fermenting yeast Pichia stipitis h c e t o i 1,2,8 3,8 4 ´ 1,5 3 3 b Thomas W Jeffries , Igor V Grigoriev , Jane Grimwood , Jose M Laplaza , Andrea Aerts , Asaf Salamov , e r 4 3 3 3 6 7 u Jeremy Schmutz , Erika Lindquist , Paramvir Dehal , Harris Shapiro , Yong-Su Jin , Volkmar Passoth t a n Paul M Richardson3 / m o c . e Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass r u t to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of a n w. xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose w metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The w / / sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary : p t insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. t h The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting p u xylose under microaerobic conditions. o r G g n i Xylose is a five-carbon sugar abundant in hardwoods and agri- RESULTS h s cultural residues1, s