纤维素酶协同超声波法提取豆粕异黄酮的研究.pdfVIP

21 3 No.3 Vol.21 2007 6 Journal of Chemical Engineering of Chinese Universities June 2007 1003-9015 2007 03-0370-05 1 1 1,2 , , 1. , 400050; 2. , 400065 8.34 mg?g? 151.63% O622.4 TQ028.96 A Extraction of Isoflavones from Soy Residue under the Intergrated Treatment with Ultrasonic and Cellulase 1 1 1,2 WANG Wan-neng , QUAN Xue-jun , LU Tian-jian 1. Collage of Bioengineering, Chongqing Institute of Technology, Chongqing 400050, China; 2. Chongqing Academy of Chinese Material Medics, Chongqing 400065, China Abstract: The process under intergrated treatnent with ultrasonic of ultrasonic and cellulase both together or each separately to promote the extraction of isoflavones from soy residue was studied. The microscopic photographs show that both ultrasonic and cellulase can destroy the cell wall of the soy residue and facilitate the diffusion of isoflavones. The extraction results show that both the processes of ultrasonic and cellulase can promote the extracting rate of the isoflavones, and when using the ultrasonic and cellulase together, the amount of the extracted isoflavones can increase to 8.34 mg per gram of soy residue, and comparing with the amount extracted without taking any measure of ultrasonic or cellulase, the increasing ratio is about 51.83%. It was concluded that the processes of ultrasonic and cellulase can be used to promote the extraction of isoflavones from soy residue effectively. Key words: soybean; isoflavones; ultrasonic; cellulase 1 [1~3] 2.37[4] [5] 2005-06-23 2005-11-27 7855 1973- E-mail wannengw@cqit.edu.cn 21 3 371 [6] 2 2.1 Puerarin 10000 U?g? 1 JY92-II AL204 TU-1901 ME1000130 TGL-16B DSHZ-300 FZ102 20~80 SPSS11.0 2.2 2.2.1 [7] 250 nm TU-1901 190~400 nm 250 nm 250 nm 5.30 mg 95% 100 mL53 μg?mL? 1 0.20 mL0.40 mL0.60 mL 0.80 mL1.00 mL 10 mL 95 1.0 mL10 mL250 nm SPSS11.0 [8] 2.2.2 2.2.1 Y mg?g? 1 2.2.3 60 3.0 g A 13 mL pH 4.550 30 minpH 7.08