活性炭固定耐冷菌对硝基苯的降解特性.pdfVIP

中国环境科学 2009,29(9) ：941~945 China Environmental Science 活性炭固定耐冷菌对硝基苯的降解特性 孙 凌 1,2,李 轶 1,3,胡洪营 1* (1.清华大学环境科学与工程系,北京 100084 ；2. 中国矿业大学(北京)化学与环境 工程学院,北京 100083 ；3.河海大学环境科学与工程学院,浅水湖泊综合治理与资源开发教育部重点实验室,江苏 南 京 210098) 摘要：研究了用颗粒活性炭固定耐冷菌(Pseudomonas putida)对硝基苯降解的强化作用.结果表明,细菌被活性炭固定后,对硝基苯的降解能 力提高.降解浓度为 200mg/L 的硝基苯所用时间由固定前的 34h 左右缩短为 29.5h.浓度为 400mg/L 的硝基苯,对细菌有强烈抑制作用,细菌 无法生长,但固定化细菌可直接降解浓度为 400mg/L 的硝基苯,且降解浓度为 600mg/L 的硝基苯只需 46h. 固定化细菌可连续4 次降解浓度 为 400mg/L 以下的硝基苯,但随着次数增多,降解速率减慢.用 SEM 扫描 200mg/L 硝基苯降解末期的生物活性炭的结果表明,Pseudomonas putida 菌是降解过程中唯一的细菌. 关键词：Pseudomonas putida ；硝基苯；颗粒活性炭；固定化；连续降解 中图分类号： 文献标识码： 文章编号： - - - X172 A 1000 6923(2009)09 0941 05 Biodegradation of nitrobenzene by a strain immobilized on granular activated carbon. SUN Ling1,2 ,LI Yi1,3, HU Hong-ying1* (1.Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China ； 2.School of Chemical and Environmental Engineering, China University of Mining and Technology(Beijing), Beijing 100083, China；3.Key Laboratory of Integrated Regulation and Resource Department on Shallow Lakes, Ministry of Education, College of Environmental Science and Engineering, Hohai University, Nanjing 210098, China). China Environmental Science, 2009,29(9)：941~945 Abstract ：Effects of nitrobenzene biodegradation enhanced by Pseudomonas putida immobilized on granular activated carbon (GAC) was studied. The biodegradation time was shortened for 200mg/L nitrobenzene from approximately 34h to 29.5h with biological activated carbon (BAC) present. Nitrobenzene with concentration at 400mg/L could not been directly biotransformed by the bacteria due to the intense inhibition before the bacteria was immobilized within