黄曲霉毒素解毒酶项目介绍.课件 - 广州科仁生物工程有限公司.pptVIP

黄曲霉毒素解毒酶项目介绍Aflatoxin-detoxifizyme Application Development 广州科仁生物工程有限公司 Guangzhou Co-win Bioengineering Co., Ltd 黄曲霉毒素（Aflatoxin，AFT）What is AFT? 是一类由黄曲霉和寄生曲霉产生的真菌毒素； A kind of mycotoxin produced by Aspergillus flavus and Aspergillus parasiticus Speare 已发现B1、B2、G1、G2、M1、M2等十几种，其中以黄曲霉毒素B1（AFB1）的毒性最强. Including Aflatoxin B1,B2,G1,G2,M1and M2 etc, among which AFB1 is the most toxic. AFT的污染广泛AFT contamination is wide-spread 黄曲霉和寄生曲霉适宜在温暖潮湿的环境下生长并产生毒素，广泛污染饲料、粮食和食品，例如花生、玉米、大米、小麦、牛奶等。 Aspergillus flavus prefer warm and moist conditions to grow and produce toxins; Most feed and food could be contaminated, such as peanut, corn, rice and milk. AFT的毒性巨大 AFT is very toxic 被国际癌症研究机构（IARC）列为天然存在的1类（Group-A）致癌物，人类摄入被污染食品可诱发原发性肝癌、胃癌、肺癌等。 AFT had been classified as carcinogen of Group A by IARC, could cause human gastric carcinoma, Iiver cancer and lung cancer etc. 毒性(toxicity)是氢氰酸的10倍，砒霜的68倍。 Toxicity of AFT is 10 times of hydrocyanic acid and 68 times of arsenic. 0.294毫克/公斤剂量的AFB1就能引起敏感动物的急性中毒死亡。 0.294mg/kg AFB1 could cause susceptive animals die of acute toxicity AFT毒性的危害Ways for AFT to harm human beings 各国食品中AFT含量的标准 Standards of AFT in Food 我国的AFT污染情况AFT contamination in China 污染广泛，形势严峻 Wide-spread and Serous 2004年，我国31个储藏稻谷样品检测真菌毒素含量，结果显示AFT阳性率达 90.32%。 In 2004, AFT contamination ratio of 31 examined stored paddy samples is 90.32% 1999年，韶关钢铁集团花生油调查,AFT大于20 ?g/kg的检出率为20.7%。 In 1999, among examined peanut oil, percentage of samples with AFT concentration above 20 μg/kg is 20.7% 2000年，某地区市售部分有品牌酱油抽样检查,结果：样品30个，AFB1呈阳性10个。 In 2000, results of a spot check of 30 samples of soy sauce for certain brand showed that 10 of 30 was AFT positive. 过往常用的AFT 去毒方法Common Methods to detoxify AFT 一、物理方法（physical） 1.筛选法(pick out) 2.漂洗法(rinse)　3.热处理法(heat) 局限性： 去毒不彻底；经过处理的食物保存时间短 ，易产生二次污染 Shortage: not thoroughly; shorter reserve time, easy to secondary contamination 二、化学方法（chemical） 1.氨化处理(amination) 2.粘土或沸石处理(clay or zeolite) 3.生石灰处理(calces) 局限性：成本高；去毒不彻底 Shortage: hi