紧张型头痛血浆NO、NOS含量变化临床和实验研究.pdf

复方羊角颗粒组和 NOS 抑制剂组注射前、后比较均有显著性差异(P＜0.05)，说明这 三种药物均可使疼痛潜伏期延长。 （3 ）与注射前比较，模型组疼痛阈值明显降低 (P ＜0.05)，其余各组均无明显差 异（P ＞0.05 ）。与对照组相比，模型组注射后的疼痛阈值小于对照组（P0.05 ）。 （4 ）柔筋方组与模型组相比较，注射后JOR 潜伏期与组别之间负相关有统计学 意义(偏相关系数=-0.478,P 0.05) ，表明柔筋方组紧张型头痛模型大鼠头区肌肉筋膜 潜伏期较模型组延长，柔筋方可以降低紧张型头痛模型大鼠头区肌肉筋膜的敏感性。 结论： (1)头区肌筋膜的痛敏感性增加在紧张型头痛的致病机制中可能起着重要 作用。 （2 ）NO 、NOS 与紧张型头痛的发病密切相关，通过影响头区肌肉和皮肤的痛 阈值，头区肌筋膜痛敏感性来参与紧张型头痛的发作。 （3 ）柔筋方汤剂能有效解除或缓解头痛，改善患者的临床症状，疗效确切。 （4 ）柔筋方汤剂调节体内血浆、脑组织中 NO 、NOS 的含量对紧张型头痛发挥 作用是其作用机制的一个方面。 关键词 柔筋方；紧张型头痛；一氧化氮/一氧化氮合酶;张颌反射 Clinical and Experimental Research on NO and NOS Changes in Blood of Tension-type headache Speciality:Internal Medicine of TCM Author:QuanWei Tutor:Chief Physician Hu Zhiqiang Abstract Objective: Quantify the intensity of noxious stimulation by neural electrophysiology, access to analysis skin、muscle and fascia’s pain threshold and sensitivity of rat model of tension headache precisely. To detect the plasma NO/NOS levels of patients with tension-type headache and rat model of tension headache, access to research the relationship between NO/NOS and TTH.To study the Roujin Decoction ’s influence on the plasma NO/NOS levels of rat model of tension headache, access to get the exact therapeutic mechanism of action. Methods: Animal experiments: Modeling method is both semispinal neck intramuscular injections of ATP. Observed amplitude and latency of rats’ JOR before and after administration. After the test,blood from abdominal vein of all rats to detect the plasma NO/NOS levels.Made 10% brain homogenate from the left brain to detect the NO/NOS levels. clinical trials 1: Select 41 patients with CTTH, blood to detect