山东省猪瘟血清学调查及其Ems基因序列分析的研究.pdf

目录 摘 要……………………………………．．…………………………………………………………………………………l Abstract．：………………………………．……………………………………………………………………………………．：； 英文缩写词表……………………………………………………．：……………………………_…．．5 ● 文献综述…………………………………………………………………………………………6 试验一山东省规模化猪场猪瘟监测与分析……………………………………………………．20 1材料与方法……………………………………………………．．………………………………20 2结果与分析………………………………………………………………………………………………………………23 3讨论………………………………………………………………………………………………………………………．24 试验二猪瘟病毒分离鉴定……………………………………………………………………一26 1材料与方法………………………………………………………………………………………………26 2结果与分析…………………………………………………………………………．…………………………………．．30 3讨论………………………………………………………………………………………………………………………．33 试验三猪瘟分离株E吣基因主要抗原编码区序列的克隆及分析………………………………34 l材料与方法………………………………………………………………：…………………………．．34 2结果与分析……………………………………………………………………………………………………………．．39 3讨论………………………………………………………………………………………………………………………．43 结论…………………………………………………………………………………………………………………………．45 参考文献……………………………………………………………………………………………46 致谢………………………………………………………………………………………………．．………………………52 Abbreviationwords table……………………………………………………………………………………………·………5 Summary………………………………^…………………………………………………………………………………………6 1 Thedetectionand ofCSFin farmsof analysis Large-scalepig Shandong……………………………一20 1．1Materialsand Methods…………………………………………………………………………………………………20 1．2Resultsand Analysis……………………………………………………………………………………………………23 1．3Discussions………………………………………………………………………………………………………………．．24 2lsolationandIdentification ofCSFV………………………………………………………………………………．26 2．1Materialsand Methods…………………………………………………………………………………………………26 2．2Resultsand Analysis…………………………………………………………………··………………………………·30 2．3Discussions………………………………………………………………………………………………………………”32 3 and of oftheIsolatedCSFV………………………………………·34 CloningSequenceanalysis Er“gene 3．1Materialsand Methods…………………………………………………………………………………………………34 3．2 Resultsand Analysis…………………………