气相色谱-联质谱法快速测定禽蛋中203种农药及化学污染物残留.pdfVIP

摘 要 摘 要 随着经济发展和人们生活水平的不断提高，人们对食品安全问题越来越重视。农药对食 品的污染是影响食品安全的主要因素，也是各国衡量食品卫生和质量安全的首要指标。同时， 世界各国在食品进出口方面对农药残留的要求越来越严格，因此建立快速、简单、准确的农 药检测方法是保证食品安全的首要任务。本文初步建立了气相色谱- 串联质谱法测定禽蛋中农 药及化学污染物残留的分析方法。全文共分为2 章： 第 1 章 本章介绍了农药、农药的分类、农药的中毒机理和农药残留及其对人体健康的 危害。重点综述了食品农残常用的前处理技术、检测方法及其原理和应用。 第 2 章 本章主要阐述了气相色谱- 串联质谱法快速测定禽蛋中203 种农药以及化学污染 物残留。比较了4 种除水实验方案、8 种提取溶剂、9 种净化固相萃取柱 （包括SPE 柱对农药 的吸附实验，SPE 柱的净化效果实验以及SPE 柱对农药的回收率实验）以及5 种洗脱溶剂对 样品的提取净化效果。最终确定了用15 mL 乙腈提取，C18 + Carb 串联固相萃取柱净化，20mL 乙腈- 甲苯 （3 ：1，v/v ）洗脱，GC-MS/MS 检测的实验方法。本方法的检出限为0.03 g/kg～ 181.9 g/kg，其回收率在60%～120%的有183 种,占研究总数的90.1% ；相对标准偏差20% 的有197 种,占研究总数的97.0% 。 关键词 气相色谱- 串联质谱 禽蛋 农药及化学污染物 I Abstract Abstract With economic development and continuous improvement of living standards, people pay more and more attention to food safety issues. Pesticide contamination of food is not only the main factor affecting food security, but also the primary indicator for many countries to measure the quality of food hygiene and safety. At the same time, more and more strict requirements have been put on the pesticide residues in food import and export, so establishing a rapid, simple and accurate detection of pesticides is the primary task of ensuring food safety. In this thesis, a gas chromatography - tandem mass spectrometry method has been established for the analysis of 203 kinds of pesticide residues in eggs. The thesis is divided into two chapters: Chapter 1 This chapter describes pesticide classification, mechanism of pesticide poisoning and pesticide residues and pesticide residues on human health hazards. Commonly used sample preparation methods and detection methods for the analysis of pesticide residues in food are reviewed. Chapter 2 This chapter describes the gas chromatogr