响应面分析法优化海地瓜酶解工艺和其抗氧化活性的研究.pdfVIP

276 Acta Nutrimenta Sinica,Jun.,2010, Vol.32 No.3 响应面分析法优化海地瓜酶解工艺及 其抗氧化活性的研究 侯付景，金春华，董明敏，李妍妍，邵亮亮，苏秀榕 （宁波大学生命科学与生物工程学院，宁波 315211） 【摘 要 】 目的 为了充分发挥海地瓜的生理功能，优化酶解工艺。方法 本研究选用复合蛋白酶，以羟自由基清 除率为标准，利用响应面分析法（RSM）优化水解条件。将最优条件下制得海地瓜水解液，igICR 小鼠，42d 后，测定 小鼠血清和肝脏组织匀浆液中 SOD，GSH-Px 活力和 MDA 含量。结果 研究结果显示：最佳的水解条件为：复合蛋白 酶加酶量 2.02%；酶解温度 51.18℃；酶解时间 127.81min，此时羟自由基清除率为 73.10%。海地瓜水解液使小鼠血清 和肝脏组织中的 SOD，GSH-Px 活力明显提高，MDA 含量显著降低。结论 该条件下海地瓜酶水解液具有良好的抗氧 化作用。[营养学报，2010，32（3）：276－280] 关键词： 海地瓜，响应面，酶解，抗氧化 中图分类号：R151.1 文献标识码：A 文章编号：0512-7955（2010）-03-0276-05 STUDY ON OPTIMIZAT ION OF ENZYMOLYSIS TECHNOLOGY OF ACAUDINA LEUCOPROCTA BY RESPONSE SURFACE METHOD AND THEIR ANTIOXIDANT ACTIVITIES ， ， ， ， ， HOU Fu-jing JIN Chun-hua DONG Ming-min LI Yan-yan SHAO Liang-liang SU Xiu-rong (Faculty of Life Science and Biotechnology，Ningbo University ，Ningbo 315211 ，China) 【 】 Abstract Objective To optimize the enzymolysis condition for Acaudina leucoprocta to research lencoprocta the antioxidant activity of its hyoholysate in vivo. Method Response surface methodology (RSM) was employed to optimize the hydrolysis conditions with protamex. The ICR mice were given ig the hydrolysate of Acaudina lencoprocta obtained under optimal conditions. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of MDA in blood serum and liver in mice were analyzed. Results and Conclusion The best hydrolysis conditions were ：the amount of enzyme was 2.02%; enzymolysis temperature was 51.18 ℃; enzymolysis time was 127.81min, hydroxyl radical scavengi