猪thrsp基编码区多态性及其与猪产脂能力的关联性.pdfVIP

摘 要 甲状腺激素应答Spot14 (THRSP) 是甲状腺激素诱导的核内蛋白，对动物脂肪生 成具有重要的调控作用。为了探明中国地方猪品种（脂肪型）和引入品种（瘦肉型） 胴体脂肪沉积之差异的遗传机理，本研究提取脂肪型猪（皖南花猪、绩溪黑猪、定远 猪，N=228 ）和瘦肉型猪(长白猪、大白猪、杜洛克及其杂种猪，N=92) 的耳组织DNA ， 检测thrsp 基因编码区的SNP，分析其变异对mRNA 折叠和蛋白质二级结构的影响以 及在群体中的分布规律，研究其变异与猪产脂能力之间的关联性。结果发现猪 thrsp 基因编码区的核苷酸序列与人和牛的同源性分别为86%和88%。存在2 个SNPs 位点 （G123A 和A308G ）分别位距CDS 起点的 123bp 和308bp 处，其中G123A 为同义 突变，而A308G 导致THRSP 蛋白质103 位的赖氨酸变为精氨酸，引起了酪蛋白激酶 Ⅱ磷酸化位点由TKEE 转变为TREE，并产生了2 种类型的mRNA 折叠和蛋白质二 级结构。脂肪型猪群中123G 和308A 的频率分别为0.9759 和0.5899，瘦肉型猪群的 123G 和308A 频率分别为0.6577 和0.8152；脂肪型猪 123G308A 和 123G308G 配子 的总频率达到 0.97 以上，而瘦肉型猪的 123A308A 和 123G308A 配子的总频率为 0.8153 。结果提示，thrsp 基因编码区的G123A 和A308G 位点多态性与猪脂肪生成能 力密切关联，对脂肪生成相关基因表达可能具有重要的调节作用。 关键词：猪，thrsp 基因，SNP，脂肪沉积 I Abstract Thyroid hormone responsive spot 14 (THRSP) is a nucleoprotein induced by thyroid hormone, which makes an essential effect on the regulation of animal lipogenesis. In order to clarify the genetic mechanisms of the differences between Chinese native pig breeds (fat-type group, FTG) and introduced pig species (lean-type group, LTG) in carcass fat deposition, we extracted DNA samples of ear tissues from FTG (n = 228) composed of Wannan Spotted, Jixi Black and Dingyuan and LTG (n = 92) from Landrace, Large White, Duroc and their hybrid pigs and then detected the SNPs in the coding region of thrsp gene. The effects of SNPs on mRNA folding and protein secondary structure in FTPG and LTPG were predicted using Vienna RNA folding software and YASPIN web services, and their distribution and association with lipogenesis capability in FTG and LTG were analyzed. The results showed that the homologies of nucleotide sequence in coding region of pig thrsp gene with human and cattle were 86% a