2010体外星形胶质细胞去分化异质性证据.pdfVIP

Cell Mol Neurobiol (2010) 30:483–491 DOI 10.1007/s10571-009-9474-3 ORIGINAL RESEARCH Evidence for Heterogeneity of Astrocyte De-Differentiation in vitro: Astrocytes Transform into Intermediate Precursor Cells Following Induction of ACM from Scratch-Insulted Astrocytes Hao Yang • Xin-Hong Qian • Rui Cong • Jing-wen Li • Qin Yao • Xi-Ying Jiao • Gong Ju • Si-Wei You Received: 5 September 2009 / Accepted: 20 October 2009 / Published online: 3 November 2009 Springer Science+Business Media, LLC 2009 Abstract Our previous study definitely demonstrated that increased with prolonged culture period ranging from 1 to the mature astrocytes could undergo a de-differentiation 10 days. Meanwhile, the protein level of A2B5 in cells also process and further transform into pluripotential neural increased significantly. These results revealed that not all stem cells (NSCs), which might well arise from the effect astrocytes could de-differentiate fully into NSCs directly of diffusible factors released from scratch-insulted astro- when induced by ACM, rather they generated intermediate cytes. However, these neurospheres passaged from one or more restricted precursor cells that might undergo pro- neurosphere-derived from de-differentiated astrocytes gressive de-differentiation to generate NSCs. possessed a completely distinct characteristic in the dif- ferentiation behavior, namely heterogeneity of differentia- Keywords Astrocytes De-differentiation tion. The heterogeneity in cell differentiation has become a Intermediate precursor cell A2B5 NG2 crucial but elusive issue. In this study, we show that Neural stem cell Cell culture purified astrocytes could de-differentiate into intermediate precursor cells (IPCs) with addition of scratch-insulted astro