RNAi技术沉默Survivin基因抑制肺癌细胞株H1299增殖并诱导其凋亡拉.pdfVIP

内科急危重症杂志2015年第2l卷第4期 鬻鬻鞘黼黼囊蓁霈囊霾雾霾嚣骥囊蘩熏霪囊囊蓁蘩震垂震簇嚣li囊蘩震囊蓁篡篓垂鬟囊嚣黧羹鬟蘩霾雾鬟薹鍪蕤黼震粪冀鬻鬻| 299 RNAi技术沉默Survivin基因抑制肺癌细胞株H1 增殖并诱导其凋亡★ 枝江市人民医院刘霜莉 舒榕h，枝江443200 摘要 h后，细胞 的Survivin基因表达明显减少，空白对照组Survivin 0．01)；细胞抑制率和凋亡率增加，转染48 Survivin 关键词 siRNAH1299细胞凋亡 中图分类号R734．2 文献标识码A DOI10．1 1768／nkjwzzz SurvivinsiI州Ainhibits andinduces ofhumancancerH1299cellsⅡU special proliferation apoptosis lung Shuang— K．sHURong”．仉People§HospitalofZhijiangCity。Zhijiaag443200．China Abstract tIle on evaluateeffectofsurvivin siRNA and ofhuman Objective：To special proliferationapoptosis lung cancerH1299 vectorof wastransfectedintoH1299cells．TheandmR— cells．Methods：TheexpressionpgsiRNA—sur protein NA ofsurvivinWasdetectedWesternblotandrealtimePCR．The ofcell andcells expression by changescycle proliferation were FCM examined and H1299 andmRNAlevelsofsurvivin by MrFl,assay，respectively．Results：Incells。theprotein were decreasedafter mRNAofcontrolteamiS controlteamiS significantly transfection，survivin 1．0，siRNA．blank 0．98． siRNA．SurteamiS reductionof wasrelatedtoanincreaseinthefractionofG2 0．32(P0．01)，and proliferation phase． survivinmRNAofcontrolteamiS teamiS50．1％after48hourof 23．7％，siRNA—Sur ratioof inhibitand ofH1299were