镶嵌纳米级再生丝素蛋白聚乙烯醇膜的制备和促细胞生长性能.pdfVIP

27 6 Vol. 27 No.6 2010 12 Journal of Biomedical Engineering December 2010 * 陈忠敏 郝雪菲 范 开 (重庆理工大学 化学与生物工程学院, 重庆 400050) : , (SFP) , ( PVA) (SFP in PVA) , 60%NaSCN , ,, SFP 80~ 150 nm, SFP/ PVA ; SFP PVA SFP/ PVA 10/ 9020/ 8030/ 7040/ 60 , CH ( MTT ) , ,SFP/PVA 30/70, CH : ;; ;; TS141.921 A 2010 Preparation of Polyvinyl lcohol Film Inlaid with Silk Fibroin Peptide Nanoscale Particles and Evaluation of Its Function to Promote Cell Growth Chen Zhongmin Hao Xuefei Fan Kai ( Col l ege of Chemi cal and B ioeng ine eri ng , Chong qi ng Univ ers i ty of T ech nol ogy , Chongq i ng 400050, Ch ina) bstract: Nanoscale particles of silk fibroin peptide ( SFP) were prepared from discarded materials of cocoon or fila ture by dissolving and enzymolysis. Polyvinyl Alcohol films inlaid with silk fibroin peptide nanoscale particles (SFP in PVA) were prepared by blending nanoSFP and PVA in water according to different blending ratios. T he films characteristics and their promoting cell growth functions were investigated. Silk fibroin fiber was dissolved in 60% NaSCN solution,and was decomposed with Chymotrypsin, Trypsin and Neutral, respectively. The uniformity of size of SFP nanoparticles prepared by Neutral was better and appeared about 80150nm. ( SFP in PVA) films were characterized by infrared spectroscopy( IR) measurement which demonstrated the combination of SFP and PVA. Scanning electron microscopy revealed the PVA films already inlaid with SFP microsegment. The surface and form stability in water of the ( SFP in PVA) films with blending ratios of 10/90, 20/ 80, 30/70 and 40/