2015_中西医结合临床专业论文03750.docVIP

Shaanxi University of Chinese Medicine 硕士学位论文 疏筋解毒汤干预PD大鼠黑质内质网 应激的实验研究 中文摘要 目的： 探讨疏筋解毒汤对帕金森病(PD)模型大鼠脑内黑质多巴胺能神经元内质网应激信号传导蛋白基因表达的调节作用。 方法： 采用向大鼠黑质内定位注射6-OHDA法制备PD大鼠模型。用正常SD大鼠作为空白对照组，并将造模成功的大鼠分为模型组、西药治疗组、疏筋解毒汤小剂量治疗组、疏筋解毒汤中剂量治疗组、疏筋解毒汤大剂量治疗组，共6个组。利用逆转录聚合酶链反应（RT-PCR）法对各实验组大鼠黑质的CHOPmRNA、XBP-1mRNA表达进行分析，检测各个指标在各组中的表达情况。 结果： 1、CHOPmRNA表达：中药组CHOPmRNA的表达从14d到60d的用药过程中，随时间的延长，CHOPmRNA的表达呈渐降趋势。且在14d、、、、Purpose: Probing the adjusting effect of shujinjiedu Tang to the gene expression of the protein in the conduction of stress signal of substantia nigra dopaminergic neuron’s endoplasmic reticulum in the model rats infected by parkinsons disease. Methods: 6-OHDA is injected into the substantia nigra of rats to make the model rats. The normal SD rats are used as the blank control group. Also, the rats that are successfully modeled are divided into six groups, such as the modeling group, the western medicine treatment group, the little, medium and large dose treatment groups with shujinjiedu Tang. RT-PCR method is used to analyze the expressions of CHOPmRNA, XBP-1mRNA in the substantia nigra of each experimental group, and the expression conditions of every indicator is detected in each group. Results: 1. The expression of CHOPmRNA: between the 14th day and the 60th day after the shujinjiedu Tang is used, the expression of CHOPmRNA shows a descending tendency with the increasing of the time. Moreover, at the following five moment, such as 14th day, 21st day, 30th day, 45th day, 60th day, the expression of CHOPmRNA is lower than that of the western medicine treatment group and the modeling group, which has certain statistical significance. And the expression of CHOPmRNA in blank control group is lower than that of the other groups at the above five moments. 2. The expression of XBP-1mRNA: between the 14th day and the 60th day after the shujinjiedu Tang is used, the expression of XBP-1mRNA shows a increasing tendency with the increasing of the time. Moreover, at the following five mo