BDNFTrkB在岛叶慢性电点燃大鼠海马表达及与学习记忆的关系.docVIP

BDNF、TrkB 在岛叶慢性电点燃大鼠海马中的 表达及与学习记忆的关系 摘要： 目的 研究岛叶慢性电点燃癫痫模型大鼠的学习记忆功能，检测脑源性神经营养因子(brain-derived neurotrophic factor，BDNF)、酪氨酸蛋白激酶受体B(Tyrosine receptor kinase B，TrkB)在海马组织中表达，探讨BDNF、TRKBwestern blot方法分别检测BDNF、TB蛋白在海马组织中表达水平，用RT-PCR检测两因子基因表达水平。结果 岛叶慢性电刺激点燃成功后第1天（即0周），学习记忆能力点燃组与空白对照组、假手术组差异无统计学意义 (P＞0.052周LT为(48.87±3.42s)、EN为（2.75±0.99次）、点燃3周LT为(48.71±2.77s)、EN为（2.75±1.15次），与对照组（即点燃0周P＜0.054周P＜0.05BDNF、TB表达均在24h达到高峰（P＜0.052~3周P＜0.014周P＜0.05TrkB在海马中表达总变化趋势一致。BDNF、TrkB与岛叶点燃大鼠学习记忆功能密切相关。 关键词：岛叶；癫痫；BDNF；TRKB；海马；避暗试验；学习记忆 中图分类号： 文献标识码：A The expression of BDNF and TrkB in rat hippocampus and the relationship with learning-remembering ability in chronic electronic ignition of insular lobe. XU Wen-zhong , WANG Feng, ZHANG Pei-song ,REN Shuang-lai,QI Jiang-hua,,SUN Tao. Ningxia Medical University,Key Lab of Craniocerbral Disease, NingXia Hui Autonomous Region,Yinchuan 750004,China Corresponding author：SUN Tao，Email：suntao@nxmu.edu.cn. 【Abstract】Objective To study the expression of active Skeleton Adjustment Protein(BDNF、TB) in rat’s Hippocampus in the model of insular chronic electricity-igniting epilepsy and investigate the relation between BDNF、TB and learning-remembering ability in the epileptic rat. Methods: To divide adult SD rats randomly into model, sham and control group, respectively. The Passive avoidance test was employed to test learning-remembering ability of rats and Immunohistochemistry and western blot was used to determine the expression of BDNF、TrkB in the hippocampus and RT-PCR was exploited to check the gene expression of the two factors.Result: There is no big difference of learning-remembering ability of rats among the igniting group or control group and sham groups ( P0.05) in the first week after insular chronic ignition when passive avoidance test was examined. The increased learning-remembering ability appeared in the 2nd week (LT: 48.87±3.42s;EN: 2.75±0.99）3rd week(LT:48.71±2.77s;EN:2.75±1.15) after the ignition(P0.05).It was declined i