《E筛选生产生物柴油的海藻原料》.pdfVIP

b i o m a s s a n d b i o e n e r g y x x x ( 2 0 1 1 ) 1 e 1 1 A v a i l a b l e a t ww w. s c i e n c e d i r e c t . c o m h t t p : / / w w w . e l s e v i e r . c o m / l o c a t e / b i o m b i o e Screening of marine microalgae for biodiesel feedstock Thi Thai Yen Doan a,*, Balasubramanian Sivaloganathan b, Jeffrey Philip Obbard a,b a Division of Environmental Science and Engineering, Faculty of Engineering, National University of Singapore, E1A #02-19, 1 Engineering Drive 2, Singapore 117576, Singapore b Tropical Marine Science Institute, National University of Singapore, S2S, 18 Kent Ridge Road, Singapore 119227, Singapore a r t i c l e i n f o a b s t r a c t Article history: Biodiesel production from microalgae lipids is increasingly regarded as a more sustainable Received 9 June 2010 and feasible alternative to conventional biodiesel feedstocks derived from terrestrial bio- Received in revised form energy crops. A total of ninety-six strains of marine microalgae, with an elevated biomass 5 February 2011 productivity and intracellular lipid content, were isolated from the coastal waters of Accepted 10 February 2011 Singapore using an automated flow cytometric cell-sorting technique. Cell sorting was Available online xxx based on the two-dimensional distribution of algal cells for red fluorescence (representing chlorophyll auto-fluorescence) against forward-light scatter (representing cell size) and red Keywords: vs. green fluorescence. Twenty-one