JNK12AP1信号转导通路对PM2.5所致气道黏液高分泌的介导作用.pdfVIP

中国医科大学学报第42卷第1期2013年1月 ·23· ofChinaMedical V01．42No．1 Journal University Jan．2013 ·论著· JNKI／2一APl信号转导通路对PM2．5所致气道 黏液高分泌的介导作用 温建立，李琪，周向东 (重庆医科大学附属第二医院呼吸内科，重庆400010) 摘要 目的 研究直径≤2．5 在蛋白水平的表达量，Realtime．PCR检测MUC5ACmRNA表达量，Western 蛋白水平的表达量均显著高于后者(P0．01)，通过Western 泌。 关键词PM2．5；c-JunN端激酶；气道黏液高分泌；信号传导 中图分类号R562 文献标志码A 文章编号0258—4646(2013)01—0023—05 17．1142．007．html 网络出版地址http：／／www．cnki．net／kcms／detail／21．1227．R．201301 Induce via invitro PM2．5 MucusSecretionJNKI／2-AP-1 Pathway WEN Jian—li，LI Qi，ZHOUXiang-dong of Affdiated Medical Medicine，Second 400010，China) (DepartmentRespiratory Hospital，ChongqingUniversity，Chongqing Abstract To themechanismofPM2．5induced mucous Invitro mucous Objective hypersecretion．Methodsairway investigate airway was ratbronchial rats dividedinto3 modelestablished cellswithPM2．5stimulation．Wistarwere hyperseerection using epithehal groups：con— trol andPM2．5+SP600125mRNAand ofMUC5ACweremeasuredreal—timePCRand group，PM2．5group group．The protein by ELISA， levelsweremeasuredwestern shift usedto respectively．TheJNKI，JNl(2，p-JNKl／2 by blotting．Electrophoreticmobilityassay(EMSA)Was detectthe activitiesofactivator As withthecontrol mRNAand ofMUC5AC DNA-binding protein一1．Resultscompared group，the protein and inPM2．5 were t