丹参与白花丹参的比较研究.docVIP

Comparative study on Salvia miltiorrhiza Bge. and Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L. Speciality：Chinese materia medica Author：Li Changfeng Tutor： Professor Zhang Yongqing Abstract Objective: The morphological characteristic, photosynthesis character, seed protein electrophoresis, peroxidase zymogram of leaves, root character and root weight, the content of chemical constituent and HPLC fingerprint between Salvia miltiorrhiza Bge. and Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L. were studied systematically, which provide basic foundation for fine germplasm selection of Salvia miltiorrhiza Bge. and the quality evaluation of Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L.. Methods: The diversity of morphological character, photosynthesis character, genetic character, the character that showing yield, the content of chemical constituent and HPLC fingerprint between Salvia miltiorrhiza Bge. and Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L. were compared using the basic methods in pharmaceutical botany, medicinal plant cultivation, plant physiology and pharmacognosy. The net photosynthetic rates were measured with CIRAS－1. The plant character and the character that showing yield were measured. The protein band of seed and peroxidase band of leaves were studied by gel electrophoresis. The extractum content was determined in accordance with China pharmacopeia. The content of tanshinoneⅡA and salvianolic acid B were determined by high performance liquid chromatography (HPLC). Results:The morphological characteristic of Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L.were similar to Salvia miltiorrhiza Bge., the numerical value II of the plant height, flowering woods and the seeds quantity for the latter were higher than that of the former. The light saturation point of Salvia miltiorrhiza Bge. was higher, while its light compensation point was lower. The seed protein electrophoresis band of both Salvia miltiorrhiza Bge.and Salvia miltiorrhiza Bge