Cerebrospinal+lfuid+from+rats+given+hypoxic+preconditioning+protects+neurons+from+oxygen-glucose+deprivation-induced+injury.pdf

NEURAL REGENERATION RESEARCH September 2015,Volume 10,Issue 9 RESEARCH ARTICLE Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury 1, *, # 2, # 3 4 1 1 4 4 Yan-bo Zhang , Zheng-dong Guo , Mei-yi Li , Si-jie Li , Jing-zhong Niu , Ming-feng Yang , Xun-ming Ji , Guo-wei Lv 1 Department of Neurology, Affiliated Hospital of Taishan Medical University, Taian, Shandong Province, China 2 Department of Endocrinology, Affiliated Hospital of Taishan Medical University, Taian, Shandong Province, China 3 Department of Neurology, Shandong Taishan Chronic Disease Hospital, Taian, Shandong Province, China 4 Hypoxia Medical Institute, Xuanwu Hospital, Capital Medical University, Beijing, China Abstract *Correspondence to: Hypoxic preconditioning activates endogenous mechanisms that protect against cerebral isch- Yan-bo Zhang, M.D., bbnnbn@163.com. emic and hypoxic injury. To better understand these protective mechanisms, adult rats were housed in a hypoxic environment (8% O /92% N ) for 3 hours, and then in a normal oxygen 2 2 # These authors contributed equally to this environment for 12 hours. Their cerebrospinal fluid was obtained to culture cortical neurons work. from newborn rats for 1 day, and then the neurons were exposed to oxygen-glucose deprivation for 1.5 hours