Effects+of+Nephrolithiasis+on+Serum+DNase+(Deoxyribonuclease+I+and+II)+Activity+and+E3+SUMO-Protein+Ligase+NSE2+(NSMCE2)+in+Malaysian+Individuals.pdf

660 Biomed Environ Sci, 2015; 28(9): 660-665 Original Article Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals* 1 1,2,# 3 1 Faridah Yusof , Atheer Awad Mehde , Wesen Adel Mehdi , Raha Ahmed Raus , 4 5 Hamid Ghazali , and Azlina Abd Rahman 1. Department of Biotechnology Engineering, College of Engineering, International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia; 2. Department of Medical Analysis, Health and Medical Technical College, Baghdad 10047, Iraq; 3. Department of Chemistry, College of Sciences for Women, University of Baghdad 10071, Iraq; 4. Department of Urology, Hospital Tengku Ampuan Afzan, Kuantan 25200, Malaysia; 5. Department of Pathology, Hospital Tengku Ampuan Afzan, Kuantan 25200, Malaysia Abstract Objective Nephrolithiasis is one of the most common disorders of the urinary tract. The aim of this study was to examine a possible relationship between DNase I/II activity and E3 SUMO-protein ligase NSE2 in the sera of nephrolithiasis patients to evaluate the possibility of a new biomarker for evaluating kidney damage. Methods Sixty nephrolithiasis patients and 50 control patients were enrolled in a case-control study. Their blood urea, creatinine, protein levels and DNase I/II activity levels were measured by spectrometry. Serum NSMCE2 levels were measured by ELISA. Blood was collected from patients of the