Effects of Taishan Pinus massoniana pollen polysaccharide on the subunit vaccine of Proteus mirabilis in birds》.pdfVIP

International Journal of Biological Macromolecules 56 (2013) 94–98 Contents lists available at SciVerse ScienceDirect International Journal of Biological Macromolecules j o u r n a l h o m e p a g e : w w w. elsev /locate/ijbiomac Effects of Taishan Pinus massoniana pollen polysaccharide on the subunit vaccine of Proteus mirabilis in birds Guolin Cui, Shixun Zhong, Shifa Yang, Xuemei Zuo, Manfei Liang, Jing Sun, Jingjing Liu, Ruiliang Zhu ∗ College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China a r t i c l e i n f o a b s t r a c t Article history: Three adjuvants, namely, Taishan Pinus massoniana pollen polysaccharide (TPPPS), white mineral oil Received 19 November 2012 (WO) and propolis (PP), were added to the outer membrane protein (OMP) of Proteus mirabilis (P. mirabilis) Accepted 2 February 2013 and their effects were compared. Three hundred 1-day-old chicks were randomly divided into five groups Available online 8 February 2013 (I–V), with 60 chicks per group, and injected subcutaneously with WO-OMP vaccine (I), PP-OMP vaccine (II), TPPPS-OMP vaccine (III), OMP-only vaccine (IV) and physiological saline (V) at 3, 7 and 12 days old. On Keywords: days 3, 7, 14, 21, 28, 35, 42 and 49 after the first vaccination, the antibody titers, interleukin-2 levels (IL-2) Taishan Pinus massoniana pollen and T-lymphocyte proliferation rates in the peripheral blood as wel