Epitope analysis, expression and protection of SAG5A vaccine against Toxoplasma gondii》.pdfVIP

Acta Tropica 146 (2015) 66–72 Contents lists available at ScienceDirect Acta Tropica j o u r n a l h o m ep a g e: w w w. elsev /locate/actatropica Epitope analysis, expression and protection of SAG5A vaccine against Toxoplasma gondii Gang Lu a,# , Lin Wang a,# , Aihua Zhou b,∗ , Yali Han a , Jingjing Guo a , Pengxia Song a , Huaiyu Zhou a , Hua Cong a , Qunli Zhao a , Shenyi He a,∗ a Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People’s Republic of China b Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, Shandong University School of Medicine, Jinan, Shandong Province 250021, People’s Republic of China a r t i c l e i n f o a b s t r a c t Article history: Bioinformatics approaches were used to identify B-cell epitopes and T-cell epitopes on SAG5A protein. Received 1 December 2014 Compared to SAG1, SAG5A with good B-cell epitopes and T-cell epitopes had a potentiality to become Received in revised form 6 March 2015 a more successful vaccine against Toxoplasma gondii. Thereafter, SAG5A DNA vaccine was constructed Accepted 9 March 2015 successfully and was injected into mice with peptide to evaluate the immunoprotection. Compared to Available online 16 March 2015 the control groups, the vaccine (DNA/peptide) could induce more effective cellular and humoral immune responses in immunized mice. F