Expression of heat shock protein 27 correlates with actin cytoskeletal dynamics and contractility of cultured human bladder smooth muscle cells》.pdfVIP

Experimental Cell Research ∎ (∎∎∎∎) ∎∎∎–∎∎∎ Contents lists available at ScienceDirect Experimental Cell Research journal homepage: /locate/yexcr Expression of heat shock protein 27 correlates with actin cytoskeletal dynamics and contractility of cultured human bladder smooth muscle cells Yongquan Wang, Zhiyong Xiong, Wei Gong, Peng Zhou, Qiubo Xie, Zhansong Zhou n, Gensheng Lu n Center of Urology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China a r t i c l e i n f o a b s t r a c t Article history: Heat shock protein 27 (HSP27) is one of the most important chaperone proteins that modulates smooth Received 19 March 2015 muscle contraction. Here we investigated the effects of HSP27 expression on cytoskeleton dynamics and Received in revised form contractile function of human bladder smooth muscle cells (BSMCs) in vitro. Cultured human BSMCs 5 August 2015 were transfected with lentiviral vectors expressing either HSP27 or HSP27-siRNAs. Normal BSMCs cells Accepted 6 August 2015 and cells transfected with the empty lentivirus were used as control. Cells were then cultured on Flexcell flexible membrane dishes and mechanical stretch (14.8% elongation) was applied. The stretch caused Keywords: significant disruption of actin cytoskeletal structure and decrease in F/G-actin ratio in BSMCs with HSP27 HSP27 over-